China Antho-Rfamide CAS NO.107535-01-3 Suppliers, Factory
Antho-RFamide is a high-purity, synthetic pentapeptide neuropeptide (pGlu-Gly-Arg-Phe-NH2) originally isolated from marine cnidarians such as the sea anemone Anthopleura elegantissima and the sea pansy Renilla köllikeri, supplied as a lyophilized white powder for advanced neurobiological and evolutionary research.
As a canonical member of the RFamide family of neuropeptides, it features a signature C-terminal Arg-Phe-NH2 (RFamide) motif and an N-terminal pyroglutamate residue, making it a fundamental tool for scientists studying primitive nervous systems, neuromuscular junctions, and G-protein-coupled receptor (GPCR) signaling.
Its well-defined sequence and potent bioactivity make it an essential reference standard for academic laboratories, marine biology institutes, and pharmaceutical R&D teams focused on neurophysiology, comparative neuroscience, and the exploration of novel GPCR ligands derived from marine organisms.
Soluble in water or dilute acetic acid; insoluble in common organic solvents
Endotoxin
≤0.5 EU/mg
Biological Activity
Acts as an RFamide-type neuropeptide; modulates ion channels and neurotransmitter release; binds to G-protein-coupled receptors (GPCRs); used to study neuromuscular transmission and primitive nervous system signaling
Storage Conditions
Store at -20°C in a dry, dark, and well-sealed container; avoid repeated freeze-thaw cycles
Stock Location
Stock in USA
MOQ
1kit
1. Mechanism of Action
The primary biological mechanism of Antho-RFamide involves its specific binding to and activation of RFamide-sensitive G-protein-coupled receptors (GPCRs) located on the surface of neuronal and muscle cells in cnidarians, triggering downstream intracellular signaling cascades. Upon interaction with its target receptor, the peptide induces conformational changes that facilitate the coupling of inhibitory Gi/o proteins, which subsequently suppress adenylate cyclase activity and reduce intracellular cyclic AMP (cAMP) levels while potentially also modulating phospholipase C (PLC) pathways to influence calcium flux. The C-terminal RFamide motif, particularly the arginine and phenylalanine residues along with the critical C-terminal amide group, forms precise hydrogen bonds and hydrophobic interactions within the receptor's ligand-binding pocket, ensuring high-affinity binding and receptor specificity. This receptor activation ultimately translates into excitatory postsynaptic potentials or direct myostimulatory signals that govern muscle tone, contraction frequency, and the synchronization of behavioral responses such as feeding, defense, and locomotion in simple metazoans.
2. Product Indications
This product is specifically indicated for in vitro laboratory research, electrophysiological assays, and behavioral pharmacology studies within neurobiological, zoological, and biochemical research settings focused on invertebrate neuroscience and GPCR biology. It serves as a primary pharmacological probe for evaluating the contractile properties of isolated muscle preparations from cnidarians and other invertebrates, typically applied in dose-response experiments to determine threshold concentrations and efficacy in inducing sustained tonic contractions. Researchers utilize Antho-RFamide to investigate the structure-activity relationships of RFamide neuropeptides, to map the distribution of RFamide-immunoreactive neurons in simple nervous systems using immunohistochemistry, and to characterize the binding kinetics and signal transduction profiles of cloned RFamide receptors in transfected cell lines. Furthermore, it is indicated for use in comparative studies contrasting the functions of invertebrate and vertebrate RFamide peptides (such as FMRFamide and NPFF), for developmental biology assays examining metamorphosis and regeneration, and as a standardized reagent in drug discovery pipelines aimed at identifying novel modulators of orphan GPCRs.
3. Clinical Efficacy of Product
Although Antho-RFamide is strictly designated for research use and is not a clinical therapeutic agent, extensive in vitro pharmacological and in vivo physiological studies have demonstrated its significant efficacy as a potent excitatory neuromuscular transmitter in cnidarian models. Laboratory evaluations confirm that the peptide induces robust, dose-dependent sustained contractions in the muscular tissues of sea anemones and sea pansies, with remarkably low threshold concentrations ranging from 5 nM to 1 µM depending on seasonal and physiological variations in the tissue. Experimental applications show that at concentrations above 10^-7 M, it increases the frequency of spontaneous muscle contractions, and at higher doses (10^-5 M and above), it can trigger prolonged tonic contractions lasting up to ten minutes, effectively mimicking endogenous neural excitation. These findings underscore the efficacy of Antho-RFamide as a reliable research reagent for dissecting the fundamental principles of neurotransmission, validating GPCR assay systems, and providing a crucial evolutionary link for understanding the conservation and diversification of neuropeptide signaling systems across the animal kingdom.
4. Certificate & Quality Control
GlobalTech Co., Ltd. is committed to providing high-purity biotechnological peptide products, adhering strictly to quality standards and obtaining internationally recognized certifications. Our production partners all employ GMP-compliant production systems and possess standardized quality control processes covering raw material procurement, synthesis, purification, testing, packaging, and storage.
A rigorous quality management system
Batch traceability and stability data
Professional laboratory testing and validation
Compliance with international trade standards
5. Delivery & Shipping
We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation. We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight. We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems.
Global Technology Co., Ltd. is a modern and advanced enterprise specializing in the production and export of active pharmaceutical ingredients (APIs), peptides, and plant extracts. The company invests in and collaborates closely with domestic manufacturers holding GMP, DMF, and FDA certifications, as well as laboratories at renowned universities, to meet diverse customer needs ranging from gram-level to ton-level.
We focus on the research and development and supply of high-quality chemical products, strictly adhering to international standards and quality systems. Our products are stable in performance and reliable in purity, widely used in industrial manufacturing, scientific research, and the fine chemical industry.
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FAQ
Frequently Asked Questions
Q1
What is Antho-RFamide (CAS 107535-01-3)?
Antho-RFamide is an RFamide-family neuropeptide originally identified in cnidarians. It acts as an excitatory neurotransmitter that activates specific G-protein-coupled receptors (GPCRs), making it an essential biochemical probe for studying neuromuscular transmission and primitive nervous system signaling.
Q2
What is the purity standard and analytical method used?
Each batch of Antho-RFamide achieves a high purity level of ≥99.0%, verified via Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) and mass spectrometry, ensuring reproducibility and batch consistency for delicate assays.
Q3
How should Antho-RFamide be reconstituted and dissolved?
Antho-RFamide lyophilized powder is highly soluble in sterile distilled water or dilute acetic acid solutions. It is insoluble in typical organic solvents. Reconstituted stock solutions should be aliquoted and stored at -20°C or below to avoid degradation.
Q4
What are the proper storage and handling guidelines?
The powder should be stored sealed at -20°C in a dry, dark environment protected from moisture and direct light. Repeated freeze-thaw cycles must be avoided after reconstitution to maintain full biological potency.
Q5
What is the minimum order quantity (MOQ) and stock availability?
The minimum order quantity (MOQ) is 1 kit. The product is readily maintained in our USA warehouse inventory, allowing rapid domestic dispatch and international express delivery via DHL, FedEx, UPS, or TNT.
Q6
Can this product be used for clinical or medical treatment?
No, Antho-RFamide is intended strictly for in vitro laboratory research, physiological assays, and comparative biological studies. It is not approved for direct clinical, pharmaceutical, veterinary, or human diagnostic applications.
