Directly sourced, cold-chain stabilized compounds manufactured in accredited synthetic biology and organic synthesis facilities.
CAS 5F-MDMB-2201 (commonly cataloged under nomenclature associated with synthetic indazole- and indole-3-carboxamide derivatives, particularly methyl 2-{[1-(5-fluoropentyl)-1H-indole-3-carbonyl]amino}-3,3-dimethylbutanoate) represents a critical synthetic reference benchmark utilized in forensic toxicology, analytical reference library construction, and academic bio-affinity screening. Navigating its industrial synthesis in China requires meticulous understanding of isomeric purity, terminal fluorination stability, and strict regulatory documentation.
| Analytical Parameter | Technical Specification Metric | Laboratory Verification Standard | Industrial Quality Control Range |
|---|---|---|---|
| Chemical Systematic Name | Methyl 2-(1-(5-fluoropentyl)-1H-indole-3-carboxamido)-3,3-dimethylbutanoate | IUPAC Standard Nomenclature | Certified Single Isomer (S-enantiomer preferred) |
| CAS Registry Reference | Associated Series (Forensic Standard Benchmark) | Chemical Abstracts Service | Verified via CAS Registry Cross-Index |
| Molecular Formula & Weight | C21H29FN2O3 | 376.47 g/mol | High-Resolution Mass Spectrometry (HRMS) | Accurate Mass: ± 0.0005 Da tolerance |
| Appearance & Physical State | Crystalline Neat Solid / Off-white Powder | Visual & Optical Microscopy | Free of particulate contamination; homogeneous |
| Chromatographic Purity | ≥ 98.5% – 99.5% (Area Normalization) | Reverse-Phase UHPLC-PDA (210–280 nm) | No single unidentified impurity > 0.10% |
| Spectroscopic Confirmation | 1H-NMR, 13C-NMR, 19F-NMR, FT-IR | 400/600 MHz Cryoprobe Spectrometry | Full structural concordance against reference libraries |
Compounds within the indole- and indazole-carboxamide families supplied by accredited China chemical manufacturers are strictly synthesized, cataloged, and exported for in vitro scientific research, certified toxicological calibration, chromatographic library enrichment, and academic receptor-affinity characterization. International procurement teams are mandated to submit institutional end-user declarations (EUD) and verify national import classifications prior to commercial order execution.
Strategic demand dynamics, clinical reference requirements, and international compliance architectures driving high-purity procurement.
The continuous evolution of synthetic cannabinoid receptor agonists (SCRAs) necessitates ultra-pure primary certified reference materials (CRMs) for public health observatories, state forensic departments, and clinical toxicology laboratories worldwide. CAS 5F-MDMB-2201 serves as a high-potency benchmark for calibrating tandem mass spectrometry (LC-MS/MS) and time-of-flight (Q-TOF) instruments, establishing definitive retention windows, fragmentation pathways, and thermal degradation profiles.
Academic biomedical institutes and contract research organizations (CROs) utilize this compound in competitive radioligand binding studies and [35S]GTPγS functional assays. Researchers focus on determining functional efficacy at CB1 versus CB2 G-protein coupled receptors, charting intracellular signaling cascades, receptor internalization rates, and β-arrestin2 recruitment kinetics to understand severe synthetic toxicity mechanisms.
Global regulatory agencies—including the US DEA, European EMCDDA, and UNODC—have steadily scheduled fluorine-bearing indole-3-carboxamides. Consequently, unverified ad-hoc manufacturing has collapsed. Current commercial procurement has consolidated into accredited, highly transparent custom chemical synthesis plants capable of delivering full analytical batch documentation under rigorous export licenses.
How modernized Chinese chemical plants achieve unmatched batch uniformity, synthesis throughput, and cost amortization in advanced organic standards.
Leading Chinese fine chemical industrial clusters (located in Hebei, Jiangsu, and Shandong) control vertical domestic precursor access: from indole cores, alkyl dihalides, and methyl L-tert-leucinate building blocks. This backward integration insulates global buyers from supply disruptions and ensures competitive, stable pricing.
Terminal fluorination of the 5-carbon aliphatic chain is historically prone to incomplete conversion or excessive dehalogenation. China's top synthesis labs utilize microchannel continuous flow reactors. This precise thermal and residence time regulation prevents side-chain elimination and achieves >99% mono-fluorinated yields.
Transitioning from milligram custom synthesis to multi-kilogram analytical batches requires industrial preparative liquid chromatography (Prep-HPLC). China's industrial facilities employ automated multi-column DAC (Dynamic Axial Compression) systems, purifying batches rapidly while controlling solvent recycling economics.
Every production lot is cross-validated with in-house Bruker 400/600 MHz NMR, Agilent GC-MS/MS, and Shimadzu LC-MS equipment. Clients receive complete spectroscopic raw data (FID files, total ion chromatograms) alongside the Certificate of Analysis (COA), satisfying international academic and legal standards.
Primary scientific domains where high-purity CAS 5F-MDMB-2201 reference materials are non-negotiable prerequisites.
Modern forensic detection relies heavily on automated screening against NIST, Wiley, and SWGDRUG electronic libraries. High-purity reference material from verified Chinese facilities is systematically converted into certified mass spectra across multiple collision energies, identifying parent molecular ions [M+H]+ and hallmark fragments (such as m/z 232 indole cleavage and m/z 145 fluoropentyl losses).
Toxicologists incubate CAS 5F-MDMB-2201 with pooled human liver microsomes (pHLM) and human hepatocytes to map enzymatic biotransformation. High-purity reference powders enable scientists to definitively identify primary biomarkers, such as ester hydrolysis products (carboxylic acid derivative) and oxidative de-fluorination metabolites, which are vital for urine and blood immunoassays.
In post-mortem and clinical overdose investigations, quantifying exact biological fluid concentrations demands high-precision standard addition curves. Ultra-pure reference standards ensure multi-point linear calibration (R² > 0.999) from 0.05 ng/mL to 100 ng/mL, guaranteeing forensic integrity admissible in legal jurisprudence.
Analysis of international chemical compliance evolution, digital traceability, and the transition toward green catalytic synthesis.
Jurisdictions across North America, the EU, and East Asia are shifting from single-substance scheduling to broad analog class scheduling based on indole/indazole carboxamide core scaffolds. In response, certified suppliers in China are expanding custom deuterated and 13C-labeled isotope internal standards (e.g., 5F-MDMB-2201-d9) to deliver unambiguous analytical discrimination.
Leading Chinese fine chemical exporters are integrating cryptographic traceability into production. Every individual vial features a serialized QR matrix linked directly to its specific HPLC chromatogram, Karl Fischer moisture titration test, and mass spectrum recorded during packaging, eliminating batch mix-ups and counterfeit products.
Environmental auditing under China's national industrial modernization policies is eliminating hazardous halogenated solvents in large-scale amide coupling. Contemporary factories utilize greener peptide coupling agents (e.g., COMU/Oxyma combinations in 2-MeTHF) to drastically diminish environmental impact while boosting crude condensation yields to above 94%.
Standard operating procedure for laboratory managers, procurement heads, and research directors when engaging China chemical manufacturers.
Confirm supplier business registration, ISO 9001 certification, and facility synthesis capacities. Request representative raw chromatographic data before issuing tenders.
Lock in critical chemical parameters: target chiral enantiomeric ratio, moisture limits (<0.5%), residual solvent cutoffs (ICH Q3C guidelines), and purity threshold (>99.0%).
Submit formal Institutional End-User Declarations affirming non-human, laboratory-only usage. Coordinate import permit documentation with Chinese export clearance agents.
Ensure vacuum-sealed packaging with inert nitrogen purging, desiccants, and amber glass vials to prevent hydrolytic ester cleavage and photolytic breakdown during transit.
Detailed answers addressing chromatographic validation, synthetic stability, export paperwork, and custom batch delivery.
CAS 5F-MDMB-2201 in neat crystalline form exhibits high stability when stored at -20°C in an amber vial protected from light and moisture. Its methyl ester moiety is vulnerable to basic or acidic aqueous hydrolysis, resulting in the corresponding carboxylic acid metabolite. In solutions (such as methanol or acetonitrile for LC-MS calibration), working standards remain stable for 6–12 months at -20°C.
Accredited synthesis laboratories use multi-nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR, and 19F-NMR) to verify exact atomic connectivity and complete terminal fluorination. Chiral HPLC on polysaccharide stationary phases (e.g., Chiralpak AD-H or OD-H) is executed to calculate enantiomeric excess (ee), ensuring the final product matches the S-enantiomer standard derived from L-tert-leucinate precursors.
Every shipment includes a comprehensive Certificate of Analysis (COA) denoting batch number, synthesis date, re-test timeline, appearance, melting range, HPLC area purity, and Karl Fischer moisture content. High-resolution mass spectra (HRMS), raw NMR printouts, and a formal compliant Material Safety Data Sheet (MSDS/SDS) matching GHS criteria are supplied with each dispatch.
Yes. Leading custom synthesis plants routinely produce deuterated (e.g., 5F-MDMB-2201-d9 featuring fully deuterated tert-butyl or pentyl chains) and carbon-13 analogs. These internal standards are critical for isotopic dilution LC-MS/MS assays to correct for biological matrix ion suppression effects in forensic fluid testing.
Analytical standards are typically aliquoted into PTFE-lined screw-cap amber borosilicate glass vials (from 1 mg, 5 mg, 10 mg up to multi-gram custom packages). Vials are vacuum-sealed inside multi-layer aluminum barrier foil pouches with active silica desiccants to block ambient humidity and UV exposure during international logistics.
Established fine chemical exporters operate dedicated logistics teams skilled in hazardous materials classification, dangerous goods (DG) airfreight booking, and complete international customs documentation. Tracked international couriers and air cargo routes are supported with continuous temperature monitoring when cold-chain delivery is required.
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